Advances in Cell Culture

Contributors to Volume 1

Preface

Ross Granville Harrison

Hybrids and Viruses: Reflections on Golden Past and Less Certain Future

Text

References

Regulation of Glycopeptide Hormone Synthesis in Cell Culture

I. Eutopic and Ectopic Production of Proteins in Cell Culture as Models for Studies on Gene Regulation

II. Induction of Glycopeptide Hormone Synthesis in HeLa 65 Cell Cultures by Aliphatic Monocarboxylates

III. Differences in the Regulation of Eutopic and Ectopic Synthesis of Glycopeptide Hormone in Culture

IV. Histone Modification Mediated by Sodium Butyrate

V. Induction of Glycopeptide Hormone Synthesis by Antineoplastic Drugs

VI. Cell Cycle Analysis of Sodium Butyrate and Hydroxyurea Effects on HeLa Cells

VII. Summary and Conclusions

References

Evaluation of Antiviral Compounds in Cell Cultures with Acute or Persistent Virus Infections

I. Introduction

II. Characteristics of Antiviral Compounds Which Can Be Assessed in Vitro

III. Tests in Cultures with Acute Virus Infections

IV. Tests in Persistently Infected Cell Lines

V. Standardization of Test Procedures

VI. Summary and Conclusions

References

The Effects of Ecdysteroid Hormones on Drosophila Melanogaster Cell Lines

I. Introduction

II. Origin and General Characteristics of the Cell Lines

III. The Ecdysteroid Response

IV. The Effect of Juvenile Hormone on the Ecdysteroid Response

V. Ecdysteroid-Responsive Genes

VI. Ecdysteroid Receptors

VII. Conclusions

References

Drosophila Cells and Cell Lines

I. Introduction

II. Primary Cultures

III. Cell Lines

IV. Cell Biochemistry

V. Experiments Using Cells

VI. Summary and Conclusions

References

Propagation of Malaria Parasites in Vitro

I. Introduction

II. Culture of Invertebrate Inhabiting Stages of Malaria Parasites

III. Culture of Vertebrate Inhabiting Stages of Malaria Parasites

IV. Summary and Conclusions

References

Regulation of Plant Organogenesis

I. Introduction

II. Regulation by Manipulation

III. Regulation at the Tissue Level

IV. Regulation at the Biochemical Level

V. Regulation at the Cellular and Molecular Levels

VI. Summary

VII. Concluding Thoughts

References

Protoplasts as Vehicles for Plant Propagation and Improvement

I. Introduction

II. Isolation and Cultivation of Plant Protoplasts

III. Protoplasts in Genetic Manipulation

References

Insect Cells for Insect Virus Production

I. Introduction

II. Availability of Cell Lines or Strains

III. Suspension Culture Systems for Virus Production

IV. Substrate Systems for Virus Production

V. Factors Affecting the Virus Yield in Cell Cultures

VI. Quality of Cell Culture Produced Viruses

VII. Conclusions

References

Gastropod Cells in Vitro

I. Background

II. Establishment and Maintenance of Cell Cultures

III. Uses of Cell Cultures

IV. Prospect

V. Summary

References

Index