Animal Cell Culture Methods

Principles of Cell Culture:D. Barnes, Animal Cell Culture Equipment and Techniques.J.P. Mather, Making Informed Choices: Medium, Serum, and Serum-free Medium. How to Choose the Appropriate Medium and Culture System for the Model You Wish to Create.R.J. Hay, Cell Line Availability: Where to Get the Cell Lines You Need.C.K. Lincoln and M.G. Gabridge, Cell Culture Contamination: Sources, Consequences, Prevention, and Elimination. Establishing Cell Lines:Y. Katakura, S. Alam, and S. Shirahata, Immortalization by Gene Transfection.M.-C. Hofmann and J.L. Millan, Establishment of Mammalian Testicular Cell Lines.S. Shirahata, Y. Katakura, and K. Teruya, Cell Hybridization, Hybridomas, and Human Hybridomas.M. Westphal and H. Meissner, Establishing Human Glioma-Derived Cell Lines.R. Li, Culture Methods for Selective Growth of Normal Rat and Human Schwann Cells.C.J. Bayne, Invertebrate Cell Culture Considerations: Insects, Ticks, Shellfish, and Worms.J. Kaplan and B. Hukku, Cell Line Characterization and Authentication.Specialized Culture Techniques:J.P. Mather, Laboratory Scale-up of Cell Cultures (0.5-50 Liters).G.F. Merrill, Cell Synchronization.D.T. Loo and J.R. Tillema, Measurement of Cell Death.A. Moore, C.J. Donahue, K.D. Bauer, and J.P. Mather, Simultaneous Measurement of Cell Cycle and Apoptotic Cell Death.M. Pirity, A.-K. Hadjantonakis, and A. Nagy, Embryonic Stem Cells, Creating Transgenic Animals.Microscopy and Morphology:D.M. Phillips, Electron Microscopy: Use of Transmission and Scanning Electron Microscopy to Study Cells in Culture.S.P. Wheatly and Y.-L. Wang, Indirect Immunofluorescence Microscopy in Cultured Cells.T.K. Woodruff, Cellular Localization of mRNA and Protein: In Situ Hybridization Histochemistry and In Situ Ligand Binding.Subject Index.