Skip to main content

Carrier-Free Enzyme Immobilization

Fundamentals and Innovations in Biocatalysis

  • 1st Edition - November 1, 2026
  • Latest edition
  • Editors: Mehdi Mohammadi, Maryam Yousefi
  • Language: English

Carrier-Free Enzyme Immobilization: Fundamentals and Innovations in Biocatalysis explores innovative techniques for immobilizing enzymes without the use of inert carriers, highli… Read more

Description

Carrier-Free Enzyme Immobilization: Fundamentals and Innovations in Biocatalysis explores innovative techniques for immobilizing enzymes without the use of inert carriers, highlighting their advantages, applications, and integration into modern biocatalytic processes. This comprehensive volume provides a detailed examination of the principles, methods, and practical applications of carrier-free enzyme immobilization, making it an essential resource for advancing biocatalysis. The book begins by introducing the concept of enzyme immobilization and the transition from traditional carrier-bound methods to innovative carrier-free techniques. It discusses the fundamental principles behind carrier-free immobilization, including the types of cross-linkers used and the advantages in enhancing enzyme stability and activity.

The book also covers various methods for the structural characterization of carrier-free immobilized enzymes. With a strong focus on practical implementation, the book examines the applications of carrier-free immobilized enzymes across different industries, highlighting the pros and cons of the method. It explores novel approaches for producing carrier-free immobilized biocatalysts and provides a comparative analysis of carrier-free versus traditional carrier-bound immobilization techniques. The book also addresses the challenges associated with implementing carrier-free immobilization in industrial processes and proposes potential solutions to overcome these obstacles.

This book is an essential resource for biochemists, chemical engineers, and researchers in biotechnology who are involved in enzyme research and development. It also serves as a valuable reference for graduate students and postdoctoral fellows in fields related to biochemistry, chemical engineering, and biotechnology. Industry professionals in biotechnology, food production, environmental science, and pharmaceuticals will find this book highly beneficial for understanding and applying carrier-free immobilization methods in their processes.

Key features

  • Focuses exclusively on carrier-free immobilization techniques, providing a detailed exploration of methods that do not require the use of inactive solid carriers
  • Highlights the growing significance of carrier-free immobilization in biocatalysis, emphasizing its advantages in enhancing enzyme stability and activity without the need for inert carriers
  • Explores the compatibility of all-enzyme carrier-free immobilization techniques with emerging technologies such as microfluidic and miniaturized biocatalytic systems, showcasing their potential in modern biocatalysis
  • Addresses the practical applications, recent advancements, and challenges of implementing carrier-free immobilization in industrial processes, offering insights and solutions for researchers and industry professionals

Readership

Biochemists, chemical engineers, researchers in biotechnology, graduate students, postdoctoral fellows

Table of contents

Section 1: Fundamentals of carrier-free and carrier-bound immobilization

1. Introduction to enzyme immobilization

2. Principles of carrier-free immobilization

Section 2: Techniques for carrier-free immobilization

3. Types of cross-linkers for carrier-free immobilization

4. Cross-linked enzyme crystals (CLECs)

5. Cross-linked enzyme aggregates (CLEAs)

6. Magnetic cross-linked enzyme aggregates (mCLEAs)

7. Methods for the structural characterization in carrier-free immobilization

Section 3: Applications and case studies

8. Practical applications of carrier-free immobilized enzymes: pros and cons

9. Novel approaches for the production of carrier-free immobilized biocatalysts

Section 4: Comparative analysis and future directions

10. Comparative analysis of immobilization methods

11. Future perspectives and challenges in carrier-free immobilization

Product details

  • Edition: 1
  • Latest edition
  • Published: November 1, 2026
  • Language: English

About the editors

MM

Mehdi Mohammadi

Dr. Mehdi Mohammadi received his Ph.D. under the supervision of Prof. Habibi in 2011 from Shahid Beheshti University, Iran. In 2009, he joined the group of Prof. Guisan at the Institute of Catalysis and Petrochemistry, CSIC, Madrid, as visiting student to learn the techniques dealing with enzyme immobilization and stabilization. During 2020-2022, he joined the group of Prof. Schallmey at the TU Braunschweig as an Alexander von Humboldt research fellow. Dr. Mohammadi is currently an Associate Professor at the National Institute of Genetic Engineering and Biotechnology of Iran. His research interests lie at the intersection of chemistry and biology, focusing on the expression, immobilization, and application of enzymes as green catalysts.
Affiliations and expertise
National Institute of Genetic Engineering and Biotechnology (NIGEB), Iran

MY

Maryam Yousefi

Dr. Maryam Yousefi is an experienced Organic Chemist as associate professor at the Nanobiotechnology Research Center of Avicenna Research Institute with more than 15 years of experience in Biocatalysis and Biotransformation over 70 published papers. Expert in enzyme immobilization and using them as biocatalyst in organic synthesis, with great attention to detail and a strong ability to work under pressure.
Affiliations and expertise
Avicenna Research Institute of Beheshti University, Iran