Skip to main content
Elsevier

Colloidal Gold

Principles, Methods, and Applications

  • 1st Edition - October 28, 1989
  • Latest edition
  • Editor: M. A. Hayat
  • Language: English

Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume tr… Read more

Description

Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today.

This universal method is applicable to most microscopical systems including optical microscopy, scanning, transmission and high voltage electron microscopy, and photoelectron, photon, fluorescent darkfield and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection.

Key features

  • Principles, methods, and applications of colloidal gold methodology in cytochemistry and immunochemistry
  • Methods for preparing colloidal gold particles of different sizes
  • Protein A-gold, protein G-gold, and lectin-gold techniques
  • The use of resins and thin cryosections
  • Multiple labeling

Readership

Researchers, technicians, teachers, and students in cell biology, immunocytochemistry, histochemistry, and electron microscopy

Table of contents

The Development and Application of Colloidal Gold as a Microscopic Probe. Methods for Synthesis of Colloidal Gold. Protein A*b1Gold and Protein G-*b1Gold Postembedding Immunoelectron Microscopy. Preparation and Application of Lectin*b1Gold Complexes. Colloidal Gold for Multiple Staining. Immunogold Labeling of Ultrathin Cryosections. Colloidal Gold for Scanning Electron Microscopy. Backscattered Electron Imaging of the Colloidal Gold Marker on Cell Surfaces. Silver-Enhanced Colloidal Gold Method. Silver-Enhanced Colloidal Gold for Light Microscopy. Strategies in Colloidal Gold Labeling of Cell Surfaces and Cytoskeletal Elements of Cultured Cells. Colloidal Gold*b1Immunoreplica Method. Immunogold Double-Diffusion Method. Colloidal Gold-Labeled Agarose*b1Gelatin Microspherule Method. Colloidal Gold for Microbiological Immunocytochemistry. Immunogold Labeling of Viruses in Suspension. Plasma Membrane Localization of Proteins with Gold Immunocytochemistry. Factors Affecting the Staining with Colloi

Product details

  • Edition: 1
  • Latest edition
  • Published: October 28, 1989
  • Language: English

About the editor

MH

M. A. Hayat

Dr. Hayat has published extensively in the fields of microscopy, cytology, immunohistochemistry, immunocytochemistry, and antigen retrieval methods. He is Distinguished Professor, Department of Biological Sciences, Kean University, Union, New Jersey, USA.
Affiliations and expertise
Distinguished Professor, Department of Biological Sciences, Kean University, Union, NJ, USA

View book on ScienceDirect

Read Colloidal Gold on ScienceDirect