Current Topics in Bioenergetics

Contributors

Preface

Contents of Previous Volumes

Determination of the Proton Electrochemical Gradient Across Biological Membranes

I. Introduction

II. General Considerations

III. Determination of Δψ and ΔpH by Ion Distribution

IV. Determination of Δψ and ΔpH with Other Methods

V. Utilization of ΔμH Measurements

Conclusion

References

Application of Electron Paramagnetic Resonance in the Study of Iron-Sulfur Clusters in Energy-Transducing Membranes

I. Introduction

II. The EPR Properties of Fe-S Clusters

III. Characterization of EPR Signals in Energy-Transducing Membranes

IV. Quantification of Signals

V. Kinetic Measurements

VI. Orientation of Fe-S Clusters in Membranes

VII. Concluding Remarks

References

Recent Developments in Spin Label EPR Methodology for Biomembrane Studies

I. Introduction

II. Nitroxide Spin Labels

III. Conventional EPR

IV. Saturation Transfer EPR

V. Pulse (Time Domain) EPR

VI. Prospects for Future Applications

References

Probing Structure and Motion of the Mitochondrial Cytochromes

I. Introduction

II. An Overview of Mitochondrial Components

III. Complex III: Cytochrome bC1

IV. Cytochrome c

V. Complex IV or Cytochrome c Oxidase

VI. ADP/ATP Translocator

VII. Conclusions and Further Questions

References

Enzyme-Catalyzed Oxygen Exchange Reactions and Their Implications for Energy Coupling

I. Introduction

II. Basic Concepts and Terminology

III. Enzyme-Catalyzed Oxygen Exchanges

IV. Epilogue

References

Use of Immunological Techniques to Study Membrane Proteins

I. Introduction

II. Preparing Antibodies against Membrane Proteins

III. Applications of Antibodies to the Study of Membrane Proteins

IV. Conclusions

References

Index