Engineered Nanopores for Bioanalytical Applications
- 1st Edition - March 19, 2013
- Latest edition
- Authors: Joshua B. Edel, Tim Albrecht
- Language: English
Engineered Nanopores for Bioanalytical Applications is the first book to focus primarily on practical analytical applications of nanopore development. These nanoscale analyt… Read more
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Description
Description
Engineered Nanopores for Bioanalytical Applications is the first book to focus primarily on practical analytical applications of nanopore development. These nanoscale analytical techniques have exciting potential because they can be used in applications such as DNA sequencing, DNA fragment sizing, DNA/protein binding, and protein/protein binding.This book provides a solid professional reference on nanopores for readers in academia, industry and engineering and biomedical fields. In addition, the book describes the instrumentation, fabrication, and experimental methods necessary to carry out nanopore-based experiments for developing new devices.
Key features
Key features
- Includes application case studies for detection, identification and analysis of biomolecules and related functional nanomaterials
- Introduces the techniques of manufacturing solid state materials with functional nanopores
- Explains the use of nanopores in DNA sequencing and the wider range of applications from environmental monitoring to forensics
Readership
Readership
Biomedical Engineering; Applied research scientists / engineers involved in the applied R&D areas of bio micro-nanofluidics, genetic engineering, Micro/nanofabrication, BioNEMS, bionanomaterials; Pharmaceuticals Engineers and Biotechnology Industry Scientists working on drug discovery, drug delivery methods, safety and drug screening; Biomedical laboratory researchers working on new methods for Tissue Engineering, Stem Cells, and Regenerative Medicine technologies; BioNEMS , biosensors, and micro- nanofluidics industries
Table of contents
Table of contents
List of ContributorsIntroductionEngineered nanopores for bioanalytical applications1. Ion Transport in Nanopores1.1 Introduction1.2 Brownian motion1.3 Net transport of ions: the Nernst–Planck equation and its derivation1.4 The conductance of a pore with uncharged walls1.5 The effect of surface charge1.6 Particle translocation through nanopores—the model of deBlois and BeanReferences2. DNA Translocation2.1 Introduction2.2 Physics of a polyelectrolyte inside a nanopore2.3 Electroosmotic flow inside a cylindrically nanopore2.4 DNA inside a nanopore2.5 Capture rate and probability of successful translocation2.6 Stalling DNA in a nanopore2.7 Stalling DNA in nanocapillariesReferences3. Instrumentation for Low-Noise High-Bandwidth Nanopore Recording3.1 Introduction3.2 Components of a nanopore setup and their integration3.3 Low-current measurement techniques3.4 Bandwidth and background noise3.5 Noise filtering, sampling, and resolution3.6 Outlook: pushing the detection limitAckowledgementsReferences4. Biological Pores on Lipid Bilayers4.1 Introduction4.2 Formation: overview and experimental protocols4.3 Pore characterization: overview and experimental protocols4.4 Bacterial pore-forming toxins4.5 Bacterial porins4.6 Pore-forming peptides4.7 ConclusionReferences5. Solid-State Nanopore Fabrication5.1 Introduction5.2 Overview of materials5.3 Fabrication methods5.4 Control of pore size5.5 Surface modificationReferences6. Case Studies Using Solid-State Pores6.1 Introduction6.2 DNA and RNA translocations6.3 DNA unzipping6.4 Optical detection6.5 DNA sequencing6.6 Protein analysis with solid-state nanopores6.7 ConclusionReferencesIndex
Product details
Product details
- Edition: 1
- Latest edition
- Published: March 29, 2013
- Language: English
About the authors
About the authors
JE
Joshua B. Edel
Affiliations and expertise
Imperial College, London, UKTA
Tim Albrecht
Affiliations and expertise
Imperial College LondonView book on ScienceDirect
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