Enzymes in RNA Science and Biotechnology

Reverse transcriptase Part I (discovery, preparation, general utilization)1. MarathonRT for routine RT-PCR and for cDNA synthesis on challenging RNA templates Structured RNAs, repeat RNAs and moreAnna Marie Pyle and Li-Tao Guo2. Engineering TNA Polymerases Through Iterative Cycles of Directed EvolutionJohn ChaputReverse transcriptase Part II (RNA structure mapping and determination)3. RNA G-quadruplex (rG4) structure detection using RTS and SHALiPE assaysChun Kit Kwok4. tRNA Structure-seq in vivo and in dropletsPhilip C. Bevilacqua5. Capture the in vivo intact RNA structurome by CAP-STRUCTURE-seqYiliang Ding6. Sequencing-based analysis of RNA structures in living cells with SHAPE-MaPDanny IncarnatoRNA polymerase (discovery, preparation, development/engineering and application)7. Making RNA: Using T7RNA polymerase to produce high yields of RNA from DNA templatesAnna Marie Pyle, Tianshuo Liu and Shivali Patel8. A Simple Approach to Improving RNA Synthesis: Salt Inhibition of RNA Rebinding Coupled with Strengthening Promoter Binding by a Targeted Gap in the DNACraig T. Martin9. Isolation of E. coli RNA polymerase transcription elongation complexes by selective photoreversible solid-phase immobilizationEric J. StrobelRNA ligase10. RNA ligation using vaccinia RNA ligase enzyme: high yield RNA coupling for RNA circularization, adapter ligation and other applicationsAnna Marie Pyle11. Chemical crosslinking and ligation methods for in vivo analysis of RNA structures and interactionsZhipeng LuPost-transcriptional modification enzymes 12. Genome-wide detection of dihydrouridine modifications in RNAWendy Gilbert13. BID-seq: The quantitative base-resolution method for pseudouridines in mammalian mRNA.14. Quantitative DAMM-seq maps multiple RNA methylations in tRNA and mitochondrial dsRNA15. Discovering RNA modification enzymes by a comparative genomics approachSatoshi Kimura16. Functional analysis of tRNA modification enzymes using Mutational profilingRyota Yamagami17. Fluorescent labelling of tRNA for interaction studies with RNA modification enzymesUte Kothe18. Synthesis of long RNA with a site-specific modification by enzymatic splint ligationYa-Ming HouEndo/Exo nucleases 19. Characterizing RNase L-mediated mRNA decay in single cellsJames Burke20. Characterization of RNase JBalasubramanian Gopal21. DICER cleavage assaysTuan Anh Nguyen22. Markos Koutmos23. DROSHA cleavage assaysTuan Anh Nguyen