Immuno-oncology and Immunotherapy Part E
- 1st Edition, Volume 199 - October 22, 2025
- Latest edition
- Editors: Lorenzo Galluzzi, Norma Bloy, Maud Charpentier, Jose Manuel Bravo-San Pedro
- Language: English
Immuno-oncology and immunotherapy, Part E, Volume 199 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting c… Read more
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Description
Description
Immuno-oncology and immunotherapy, Part E, Volume 199 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including an Evaluation of agents that affect anti-tumor function of CD8+ T cells at activation, Cancer cyro-immunotherapy: Models and methods, Methods for analysis of cancer- and TLS-associated immune infiltrates by multiplex immunofluorescence histology, In-vivo measurement of the extracellular ATP concentration by bio-luminescent probes, and much more.
Additional sections cover the Analysis of the tumor microenvironment using imaging mass cytometry data, Saphenous vein blood collection for different immune analyses of living mice, Use of mouse KO models to validate the specificity of monoclonal antibodies, Imaging mass cytometry to analyze the immune TME, Autoimmune arthritis: Transgenic mouse models and methods, Assessment of human and mouse tumor-antigen specific CD8+ T cells by multimer staining in multiple compartments, and more.
Additional sections cover the Analysis of the tumor microenvironment using imaging mass cytometry data, Saphenous vein blood collection for different immune analyses of living mice, Use of mouse KO models to validate the specificity of monoclonal antibodies, Imaging mass cytometry to analyze the immune TME, Autoimmune arthritis: Transgenic mouse models and methods, Assessment of human and mouse tumor-antigen specific CD8+ T cells by multimer staining in multiple compartments, and more.
Key features
Key features
- Authored by established and active cell biologists and immunologists drawn from international sources
- Includes in-depth coverage and detailed protocols
- Presents a highly specialized group of topics that delve deep into new updates and future prospects
Readership
Readership
Researcher and Scientiists, Academics and Educators, Industry Professionals
Table of contents
Table of contents
1. Evaluation of agents that affect anti-tumor function of CD8+ T cells at activation
Douglas McNeel
2. Cancer cyro-immunotherapy: Models and methods
George Prendergast
3. Methods for analysis of cancer- and TLS-associated immune infiltrates by multiplex immunofluorescence histology
Ileana Mauldin and Leon Zheng
4. In-vivo measurement of the extracellular ATP concentration by bio-luminescent probes
Mario Tarantini, Anna Lisa GIULIANI, Valentina Vultaggio-Poma, Simonetta Falzoni, Elena Adinolfi and Francesco Di Virgilio
5. Analysis of the tumor microenvironment using imaging mass cytometry data
Zlatko Trajanoski and Zuzana Loncova
6. Saphenous vein blood collection for different immune analyses of living mice
Ana Carolina Martinez Torres, Kenny Misael Calvillo-Rodriguez, Reyes Tamez-Guerra, Cristina Rodriguez-Padilla and Kassandra Ofelia Rodriguez-Aguillon
7. Use of mouse KO models to validate the specificity of monoclonal antibodies
George Prendergast, Lisa D. Laury-Kleintop, Summer Sedano and Kaylend Manley
8. Imaging mass cytometry to analyze the immune TME
Louis M. Weiner
9. Autoimmune arthritis: Transgenic mouse models and methods
Laura Mandik-Nayak, Lauren Merlo and Weidan Peng
10. Assessment of human and mouse tumor-antigen specific CD8+ T cells by multimer staining in multiple compartments
Maha Ayyoub, Clara Maria SCARLATA, Virginie Feliu and Christel Devaud
11. Generation and functional evaluation of bispecific antibodies
Luis Alvarez-Vallina, Antonio Tapia Galisteo, Anaïs Jiménez-Reinoso, Rodrigo Lázaro Gorines and Belén Blanco
12. Isolation and phenotype analysis of intraepithelial lymphocytes from the mouse colon
Laura Bracci, Alessia Fiore, Michela Tozzi, Paola Rinzo, Daniele Macchia, Massimo Spada and Alessia Fabbri
13. Analysis of single cell TCR repertoires and gene expression from multi-modal scRNA-seq data
Dietmar Rieder, Christina Plattner and Gregor Sturm
14. Analysis of the tumor immune microenvironment of brain tumors by flow-cytometry
Claire I. Vanpouille-Box
15. Assessment of in vitro interactions between eosinophils and cancer cells by imaging-enhanced flow cytometry and time-lapse microscopy.
Fabrizio Mattei, Giovanna Schiavoni, Caterina Antonucci, Adriana Rosa Gambardella and Valentina Tirelli
16. Pharmacological screening to identify potential regulators of antigen presentation by dendritic cells
Peng LIU, Liwei Zhao, Guido Kroemer, Oliver Kepp, Yuhong Pan and Shuai Zhang
Douglas McNeel
2. Cancer cyro-immunotherapy: Models and methods
George Prendergast
3. Methods for analysis of cancer- and TLS-associated immune infiltrates by multiplex immunofluorescence histology
Ileana Mauldin and Leon Zheng
4. In-vivo measurement of the extracellular ATP concentration by bio-luminescent probes
Mario Tarantini, Anna Lisa GIULIANI, Valentina Vultaggio-Poma, Simonetta Falzoni, Elena Adinolfi and Francesco Di Virgilio
5. Analysis of the tumor microenvironment using imaging mass cytometry data
Zlatko Trajanoski and Zuzana Loncova
6. Saphenous vein blood collection for different immune analyses of living mice
Ana Carolina Martinez Torres, Kenny Misael Calvillo-Rodriguez, Reyes Tamez-Guerra, Cristina Rodriguez-Padilla and Kassandra Ofelia Rodriguez-Aguillon
7. Use of mouse KO models to validate the specificity of monoclonal antibodies
George Prendergast, Lisa D. Laury-Kleintop, Summer Sedano and Kaylend Manley
8. Imaging mass cytometry to analyze the immune TME
Louis M. Weiner
9. Autoimmune arthritis: Transgenic mouse models and methods
Laura Mandik-Nayak, Lauren Merlo and Weidan Peng
10. Assessment of human and mouse tumor-antigen specific CD8+ T cells by multimer staining in multiple compartments
Maha Ayyoub, Clara Maria SCARLATA, Virginie Feliu and Christel Devaud
11. Generation and functional evaluation of bispecific antibodies
Luis Alvarez-Vallina, Antonio Tapia Galisteo, Anaïs Jiménez-Reinoso, Rodrigo Lázaro Gorines and Belén Blanco
12. Isolation and phenotype analysis of intraepithelial lymphocytes from the mouse colon
Laura Bracci, Alessia Fiore, Michela Tozzi, Paola Rinzo, Daniele Macchia, Massimo Spada and Alessia Fabbri
13. Analysis of single cell TCR repertoires and gene expression from multi-modal scRNA-seq data
Dietmar Rieder, Christina Plattner and Gregor Sturm
14. Analysis of the tumor immune microenvironment of brain tumors by flow-cytometry
Claire I. Vanpouille-Box
15. Assessment of in vitro interactions between eosinophils and cancer cells by imaging-enhanced flow cytometry and time-lapse microscopy.
Fabrizio Mattei, Giovanna Schiavoni, Caterina Antonucci, Adriana Rosa Gambardella and Valentina Tirelli
16. Pharmacological screening to identify potential regulators of antigen presentation by dendritic cells
Peng LIU, Liwei Zhao, Guido Kroemer, Oliver Kepp, Yuhong Pan and Shuai Zhang
Product details
Product details
- Edition: 1
- Latest edition
- Volume: 199
- Published: October 22, 2025
- Language: English
About the editors
About the editors
LG
Lorenzo Galluzzi
Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.
Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals:
OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.
Affiliations and expertise
Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USANB
Norma Bloy
Norma received her master’s degree in health biology from Paris Saclay. In 2013, she joined the laboratory of Dr. Guido Kroemer at the Centre de Recherche des Cordeliers (Paris, France) and at Gustave Roussy (Villejuif, France), the largest center for oncological patients in Europe. She worked on several projects linked to immunosurveillance, culminating with her obtaining her PhD in 2017 with an original work on “Immunogenic stress and death of cancer cells: Contribution of antigenicity vs adjuvanticity to immunosurveillance”. She then moved to Weill Cornell Medicine to join the program in radiation and immunity under the mentorship of Dr. Galluzzi. Her current research is focused on investigating resistance to immunotherapy in a mouse model of HR+ breast cancer.
Affiliations and expertise
Weill Cornell Medical College, USAMC
Maud Charpentier
Maud Charpentier received her M.Sc. in Cellular Biology and her Ph.D. in Immunology from the University of Nantes, France. She chose to pursue an academic career and continued her postdoctoral training in the United States. She joined the Department of Radiation Oncology at Weill Cornell Medicine under the mentorship of Dr. Sandra Demaria. Maud has a long-standing interest in the anti-tumor immune response and its role in controlling cancer progression and treatment outcomes. Her research focuses on understanding the synergy between radiation therapy and immunotherapy in solid tumors, with the aim of overcoming resistance to treatment and developing innovative therapeutic approaches in preclinical models.
Affiliations and expertise
Weill Cornell Medicine, USAJB
Jose Manuel Bravo-San Pedro
Jose Manuel Bravo-San Pedro is currently a researcher at the Department of Physiology of the Complutense University of Madrid thanks to a Ramon y Cajal contract grant. He got his Ph.D. in biochemistry, cellular biology and genetics from the University of Extremadura (Caceres, Spain) in 2011, and he did a post-doctoral stage in the laboratory of Prof. Guido Kroemer. His main research interests have always been linked to autophagy, addressing this cellular process associated with neurodegenerative diseases or cancer and recently obesity and specifically related to problems in the correct functioning of the cilium. He is co-inventor of two patents and co-author of 110 publications indexed in PubMed in prestigious international journals, with h-index 45 and 23768 cites (Dec 2022).
Affiliations and expertise
Departamento de Fisiología, Facultad de Medicina, Universidad Complutense de Madrid, Madrid, SpainView book on ScienceDirect
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