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Elsevier

Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry

  • 1st Edition - April 23, 2021
  • Latest edition
  • Author: Bindesh Shrestha
  • Language: English

Imaging mass spectrometry (MS) techniques are often utilized without an understanding of their underlying principles, making it difficult for scientists to determine when and ho… Read more

Description

Imaging mass spectrometry (MS) techniques are often utilized without an understanding of their underlying principles, making it difficult for scientists to determine when and how they can exploit MS to visualize their biomolecules of interest. Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry is an essential reference to help scientists determine the status and strategies of biomolecule analysis, describing its many applications for diverse classes of biomolecules.

The book builds a foundation of imaging MS knowledge by introducing ionization sources, sample preparation, visualization guidelines, molecule identification, quantification, data analysis, etc. The second section contains chapters focused on case studies on analyzing a biomolecule class of molecules. Case studies include an introduction/background, and a summary of successful imaging MS studies with illustrative figures and future directions.

Key features

  • Provides the introductory foundations of imaging mass spectrometry for those new to the technique
  • Organized by topic to facilitate a quick deep dive, allowing researchers to immediately apply the imaging MS techniques to their work
  • Includes case studies summarizing the imaging MS techniques developed for the class of molecules

Readership

Scientists interested in imaging mass spectrometry, including bioanalytical chemists in academia and pharma, with or without experience with mass spectrometry (either as mass spec lab scientists/PIs or customers of such mass spec services). Biochemists, biologists, biomedical scientists or other scientists who want to learn more about visualizing specific biomolecules on the tissue.

Table of contents

1. Fundamentals of Imaging Mass Spectrometry2. Ionization Source for Imaging Mass Spectrometry3. Sample Preparation and MALDI Matrix Applications4. Tissue Sectioning for Imaging Mass Spectrometry5. Matrix for Matrix-assisted Laser Desorption/Ionization (MALDI)6. Molecule Identification Approaches in Imaging Mass Spectrometry7. Quantitation Workflow in Imaging Mass Spectrometry8. Spatial Resolution of Imaging Mass Spectrometry9. Visualization of Imaging Mass Spectrometry10. Analysis of imaging mass spectrometry data11. Multimodal imaging mass spectrometry12. Validation and Standardization of Imaging Mass Spectrometry13. Towards Clinical Imaging Mass Spectrometry14. Case study: Imaging N-Glycan on tissue15. Case study: Reactive matrix and neurotransmitters and brain metabolites16. Case study: Endogenous metabolites in mammalian tissues17. Case study: Imaging of small drug molecules18. Case study: Imaging Gangliosides

Product details

  • Edition: 1
  • Latest edition
  • Published: April 29, 2021
  • Language: English

About the author

BS

Bindesh Shrestha

Dr. Bindesh Shrestha is a senior scientist at the Waters Corporation focusing on imaging mass spectrometry. He has co-authored more than two dozen peer-reviewed articles in the field of mass spectrometry spanning over the last decade. His publications have been featured multiple times on the covers of leading journals, including Angewandte Chemie, Analytical Chemistry, Analyst, and more.
Affiliations and expertise
Senior Application Support Scientist, Waters Corporation, Beverly, MA, USA

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