Microbial Synthetic Biology

Series Page

Contributors

Preface

Chapter 1. Computational Intelligence in the Design of Synthetic Microbial Genetic Systems

Abstract

1 Introduction

2 Computational Infrastructure for Synthetic Biology

3 Computational Intelligence

4 Discussion

References

Chapter 2. Constraints in the Design of the Synthetic Bacterial Chassis

Abstract

1 Introduction

2 Top-Down Versus Bottom-Up Framing

3 An Unlimited List of Functions (Organised Starting from the Cell's Structure)

4 Perspective: The Fourth Dimension (When Time Measures and Shapes)

Acknowledgements

References

Chapter 3. Social Dimensions of Microbial Synthetic Biology

Abstract

1 Introduction: Making Space for a New Discussion

2 From Implications to Dimensions

3 From Speculation to Anticipation

4 From Public Acceptance to Public Good

5 From Regulation to Governance

Conclusions

References

Chapter 4. Bacillus subtilis: Model Gram-Positive Synthetic Biology Chassis

Abstract

1 Introduction

2 The B. subtilis Genome

3 Genome Management and Analysis of Gene Function

4 Analysis of the Transcriptome

5 Analysis of the Proteome

6 Analysis of the Metabolome

7 Parts, Devices, Systems and Applications

8 Computational Tools and Resources

Summary

References

Chapter 5. Engineering Microbial Biosensors

Abstract

1 Introduction

2 Areas of Application

3 Types of Biosensors

4 Reporters

5 Biosensors and Synthetic Biology

6 Transcription-Based Biosensors

7 Translation-Based Biosensors

8 Posttranslational Biosensors

9 In Vitro Biosensors

10 Modelling Biosensors

11 Outlook

References

Chapter 6. Noise and Stochasticity in Gene Expression: A Pathogenic Fate Determinant

Abstract

1 Introduction

2 Origins of Noise

3 Measuring Noise

4 Engineering Noise

5 Noise and Heterogeneity in Gene Expression

6 Bistable Expression of Pneumococcal Pili

7 Cooperative Virulence in Salmonella Enterica S. Typhimurium

Concluding Remarks

References

Chapter 7. Platforms for Genetic Design Automation

Abstract

1 Introduction

2 Standards

3 Repositories

4 GDA Software Tools

5 Discussion

Acknowledgements

References

Index