Mitochondrial Translocases Part A

1. Isolation of yeast mitochondria by differential centrifugation
Heike Rampelt

2. Isolation of yeast mitochondria by affinity purification using magnetic beads
Pin-Chao Liao, Liza Pon, Tzu-Ying Lin and Shih-Hung Chien

3. A modified procedure for the separation of mitochondria from peroxisomes
Doron Rapaport and Nitya Aravindan

4. Subcellular fractionation for the analysis of mitochondrial proteins
Nils Wiedemann and Conny Steiert

5. Monitoring mitochondrial localization of dual localized proteins using a Bi-Genomic Mitochondrial-Split-GFP
Hubert Dominique Becker

6. Analysis of mitochondrial localization and biogenesis by genetic screens and automated imaging
Maya Schuldiner and Yury S. Bykov

7. Methods to monitor mitochondrial disulfide bonds
Agnieszka Chacinska, Ben Hur Mussulini, Michał Wasilewski and Klaudia K Maruszczak

8. Prediction of mitochondrial targeting signals and their cleavage sites
Paul Horton, Kenichiro Imai and Yoshinori Fukasawa

9. Monitoring of mitochondrial preprotein processing, peptide accumulation and degradation
Nora Vögtle and Cansu Kücükköse

10. Analysis of mitochondrial targeting signal cleavage and processing by mass spectrometry
Pitter F. Huesgen

11. Analysis of targeting signals for mitochondrial intermembrane space import
Kostas Tokatlidis

12. Analysis and prediction of internal mitochondrial targeting signals
Timo Mühlhaus, Johannes M. Herrmann, Saskia Rödl and Felix Jung

13. Monitoring and analysis of mitochondrial precursor protein aggregates in the cytosol
Agnieszka Chacinska

14. In vitro translation of mitochondrial precursor proteins in yeast extract to study interactions with cytosolic chaperones
Layla Drwesh

15. In vitro import of mitochondrial precursor proteins into yeast mitochondria
Dejana Mokranjac

16. Monitoring the import and assembly of mitochondrial precursor proteins into human cell line mitochondria
Diana Stojanovski and Jordan Crameri

17. Import of mitochondrial precursor proteins into mitochondria with semi- intact cells
Johannes M. Herrmann

18. The MitoLuc Assay for the Analysis of the Mechanism of Mitochondrial Protein Import
Ian Collinson, Hope Isobel Needs, Youmian Yan and Natalie Niemi

19. Monitoring mitochondrial protein import by live cell imaging
FX Reymond Sutandy

20. Quantification of mitochondrial protein import by mass spectrometry
Christian Münch

21. Methods to analyze mitochondrial protein translocation in plant mitochondria
Monika W. Murcha

22. In organello silencing of mitochondrial gene expression
Luis Daniel Cruz-Zaragoza and Mats Koschel

23. Assays to monitor mitochondrial translation
Martin Ott

24. Analysis of mitochondrial translation using click chemistry
Sven Dennerlein and Roya Yousefi