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Mucins

  • 1st Edition, Volume 732 - August 15, 2026
  • Latest edition
  • Editors: David Christianson, Stacy Malaker, Karen N. Allen
  • Language: English

Mucins, Volume 732 serves as a valuable resource for researchers aiming to investigate mucin biology in greater depth, offering a wide range of experimental approaches and analyt… Read more

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Description

Mucins, Volume 732 serves as a valuable resource for researchers aiming to investigate mucin biology in greater depth, offering a wide range of experimental approaches and analytical strategies. Topics in this new release cover Enrichment/isolation, glycoproteomics, Mucinase cleavage characterization and usage in glycoproteomics, Fluorescent protein-based FRET substrates for activity screening of mucinases, Development of clinical lectin assays based on MS glycomic discovery, Enzymatic selective sulfation of synthetic mucins, cryoEM, Cell-based mucin arrays and glycoengineering, CRISPR screens to characterize and identify glycan-binding proteins, and much more.

Additional sections cover MD Simulations, Interactions with pathogenic bacteria, Interactions with microbiota, Covalent crosslinking of mucins, Liquid Glycan and Liquid Lectin Array-based exploration of musin recognition, Quantification of mucins, Mucins in IBD, Investigations of mucins and innate immunity, Generating synthetic mucin glycopeptides, and Microscopy and imaging.

Key features

  • Covers a wide range of cutting-edge methodologies for studying mucins and glycoproteins
  • Integrates basic science with clinical and translational research applications
  • Features contributions from leading experts across multiple scientific disciplines

Readership

Researchers and professionals in glycobiology, mucin biology, and glycoproteomics, as well as those studying host–microbe interactions, mucosal immunology, and related clinical conditions; It will also be valuable to structural biologists, biochemists, and translational scientists seeking to apply cutting-edge methods to complex glycoprotein systems; Graduate students, postdoctoral researchers, and industry scientists will find it a comprehensive and practical resource

Table of contents

1. Enrichment/isolation, glycoproteomics
Stacy Malaker, Shane Finn and Sarah Lowery

2. Mucinase cleavage characterization and usage in glycoproteomics
Stacy Malaker, Alexandra Steigmeyer and Joann Chongsaritsinsuk

3. Fluorescent protein-based FRET substrates for activity screening of mucinases
Alisdair Boraston

4. Development of clinical lectin assays based on MS glycomic discovery
Niclas Karlsson, Ali Reza Afshari and Mary Solbego

5. Enzymatic selective sulfation of synthetic mucins
Jessica Kramer

6. cryoEM
Deborah Fass

7. Cell-based mucin arrays and glycoengineering
Yoshiki Narimatsu, Christian Bull, Henrik Clausen and Felix Goerdeler

8. CRISPR screens to characterize and identify glycan-binding proteins
Simon Wisnovsky

9. MD Simulations
Fiona Kearns

10. Interactions with pathogenic bacteria
Katharina Ribbeck

11. Interactions with microbiota
Eric Martens

12. Covalent crosslinking of mucins
Thomas Crouzier

13. Liquid Glycan and Liquid Lectin Array-based exploration of musin recognition
Ratmir Derda

14. Quantification of mucins
Sumaira Z. Hasnain

15. Mucins in IBD
Kirk Bergstrom

16. Investigatins mucins and innate immunity
Thaher Pelaseyed

17. Generating synthetic mucin glycopeptides
Peter Seeberger, Kabita Pradhan and Charlotte Völkel

18. Microscopy and imaging
Malin E.V. Johansson, George M H Birchenough, Elisabeth E L Nyström and Brendan Dolan

Product details

  • Edition: 1
  • Latest edition
  • Volume: 732
  • Published: August 15, 2026
  • Language: English

About the editors

DC

David Christianson

After completing studies for the A.B., A.M., and Ph.D. degrees in chemistry at Harvard University, David W. Christianson joined the faculty of the University of Pennsylvania, where he is currently the Roy and Diana Vagelos Professor in Chemistry and Chemical Biology. At Penn, Christianson’s research focuses on the structural and chemical biology of the zinc-dependent histone deacetylases as well as enzymes of terpene biosynthesis. His research accomplishments have been recognized by several awards, including the Pfizer Award in Enzyme Chemistry and the Repligen Award in Chemistry of Biological Processes from the American Chemical Society, a Guggenheim Fellowship, and the Elizabeth S. and Richard M. Cashin Fellowship from the Radcliffe Institute for Advanced Study at Harvard University. Christianson is also a dedicated classroom teacher, and his accomplishments in this regard have been recognized by the Lindback Award for Distinguished Teaching at Penn and a Rhodes Trust Inspirational Educator Award from Oxford University. Christianson has also held visiting professorships in the Department of Biochemistry at Cambridge University and the Department of Chemistry and Chemical Biology at Harvard University. Christianson has served with Prof. Anna Pyle as Co-Editor-in-Chief of Methods in Enzymology since 2015.

Affiliations and expertise
University of Pennsylvania, USA

KA

Karen N. Allen

Dr. Karen N. Allen works at the Department of Chemistry of the Boston University, the Metcalf Center for Science and Engineering
Affiliations and expertise
Department of Chemistry, Metcalf Center for Science and Engineering, Boston University, Boston, MA, USA