Neurons: Methods and Applications for the Cell Biologist
- 1st Edition, Volume 71 - July 8, 2003
- Latest edition
- Editors: Peter J. Hollenbeck, James R. Bamburg
- Language: English
Neurons: Methods and Applications for the Cell Biologist lays out numerous simple techniques for growing and carrying out experiments with many varieties of neurons. Subjec… Read more
Description
Description
Neurons: Methods and Applications for the Cell Biologist lays out numerous simple techniques for growing and carrying out experiments with many varieties of neurons. Subjects include peripheral and central neurons from vertebrate and invertebrate sources, as well as neuron-like cell lines. It also explains recent advances in our ability to introduce exogenous proteins and genes to neurons in culture. Procedures for successful protein infiltration, biolistic transfection, electroporation, and viral transgenic methods in neurons are also presented.
Key features
Key features
- Contains culture methodology for more than a dozen types of CNS and PNS neurons
- Includes most recent and reliable techniques from expert practitioners for specific experimental applications
- Addresses the latest strategies for transfecting neurons
Readership
Readership
Cell and Molecular Biologists, developmental biologists and neuroscientists
Table of contents
Table of contents
Comparing the Properties of Neuronal Culture Systems Growing and Working with Peripheral Neurons
Dissection and Culturing of Chick Ciliary Ganglion Neurons
The Culture of Chick Forebrain Neurons
Growing and Working with Spinal Motor Neurons
Avian Purkinje Neuronal Cultures
Culturing Hippocampal and Cortical Neurons
Working with Xenopus Spinal Neurons in Live Cell Culture
Culturing Neurons from the Snail Helisoma
The Ti1 Pioneer Pathway
Techniques to Dissect Cellular and Subcellular Function in Drosphila Nervous System
PC12 Cells as a Model for Studies of Regulated Secretion in Neuronal and Endocrine Cells and more.
Dissection and Culturing of Chick Ciliary Ganglion Neurons
The Culture of Chick Forebrain Neurons
Growing and Working with Spinal Motor Neurons
Avian Purkinje Neuronal Cultures
Culturing Hippocampal and Cortical Neurons
Working with Xenopus Spinal Neurons in Live Cell Culture
Culturing Neurons from the Snail Helisoma
The Ti1 Pioneer Pathway
Techniques to Dissect Cellular and Subcellular Function in Drosphila Nervous System
PC12 Cells as a Model for Studies of Regulated Secretion in Neuronal and Endocrine Cells and more.
Review quotes
Review quotes
"...invaluable for workers at all levels of cell biology."—NATURE
Product details
Product details
- Edition: 1
- Latest edition
- Volume: 71
- Published: October 2, 2003
- Language: English
About the editors
About the editors
PH
Peter J. Hollenbeck
Affiliations and expertise
Purdue University, West Lafayette, U.S.A.JB
James R. Bamburg
Affiliations and expertise
Colorado State University, Fort Collins, U.S.A.View book on ScienceDirect
View book on ScienceDirect
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