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Elsevier

Next Generation Sequencing (NGS) Technology in DNA Analysis

  • 1st Edition - November 30, 2023
  • Latest edition
  • Editors: Hirak Ranjan Dash, Kelly M. Elkins, Noora Rashid Al-Snan
  • Language: English

Next Generation Sequencing (NGS) Technology in DNA Analysis explains and summarizes next generation sequencing (NGS) technological applications in the field of forensic DNA analy… Read more

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Description

Next Generation Sequencing (NGS) Technology in DNA Analysis explains and summarizes next generation sequencing (NGS) technological applications in the field of forensic DNA analysis. The book covers the transition from capillary electrophoresis (CE)-based technique to NGS platforms and the fundamentals of NGS technologies, applications, and advances. Sections provide an overview of NGS technology and forensic science, including information on processing biological samples for forensic analysis, sequence analysis, and data analysis software as well as the analysis of NGS data. The book explores the valuable applications of NGS-based forensic DNA analysis and covers the validations and interpretation guidelines of NGS workflows.

With chapter contributions from an international array of experts and the inclusion of practical case studies, this book is a useful reference for academicians and researchers in genetics, biotechnology, bioinformatics, biology, and medicine as well as forensic DNA scientists and practitioners who aim to learn, use, apply, and validate NGS-based technologies.

Key features

  • Describes the fundamentals of NGS and its advances for forensic applications
  • Explains the transition from CE-based technique to NGS technology
  • Includes case studies related to NGS and DNA fingerprinting
  • Explores the future use and applications of NGS technologies

Readership

Academics and researchers in genetics, biochemistry, biotechnology, bioinformatics, biology, and medicine, forensic DNA scientists and practitioners who aim to learn, use, apply and validate NGS-based technologies Graduate students in genetics, biotechnology, bioinformatics, biology, and medicine interested in the application of NGS-based technologies

Table of contents

SECTION 1 Useful applications of NGS-based forensic DNA analysis

1. Transition of capillary electrophoresis to next generation sequencing for forensic DNA analysis: Need of the hour

2. Using conventional STR technology in analyzing biological transfer evidence

3. Overview of NGS platforms and technological advancements for forensic applications

4. Processing of biological samples for forensic NGS analysis

5. Commercial kits commonly used for NGS based forensic DNA analysis

6. Applications of nanopore sequencing for forensic analysis

7. Microhaplotypes analysis for human identification using next-generation sequencing (NGS)

8. Tools and techniques of using NGS technology in population studies

SECTION 2 Useful applications of NGS-based forensic DNA analysis

9. Applications of NGS in analysis of autosomal STRs

10. Differentiating monozygotic twins using NGS

11. Application of NGS in maternal genome analysis in ancient human remains

12. Application of NGS technology for parentage testing and relatedness analysis

13. Forensic relevance of SNP analysis in next-generation sequencing

14. Forensic analysis of hair using NGS technology

15. Forensic DNA phenotyping using next-generation sequencing

16. Forensic DNA phenotyping in the next-generation sequencing era

17. Forensic applications of epigenetic (DNA methylation) markers through NGS

18. Forensic applications of NGS-based microRNA analysis

19. NGS profiling of water and soil microbial DNA in forensic science

20. Applications of NGS in analysis of challenging samples

21. Detection of human body fluid through mRNA analysis using NGS

22. NGS-based detection and differentiation of forensically relevant body fluids using conventional, molecular, and microbial techniques

23. Application of next-generation sequencing technology and investigative genetic genealogy to cold cases

SECTION 3 Challenges in implementing NGS in forensic laboratories

24. Troubleshooting and challenges of Next-generation sequencing technology in forensic use

25. Validation of NGS for casework at forensic DNA laboratories

26. Challenges in using genetic genealogy in forensics

27. The role of artificial intelligence and machine learning in NGS

28. Ethical issues of forensic application of NGS technology

29. Ethical, legal, and social challenges of next-generation sequencing technologies (NGS) in forensic criminal identification

Product details

  • Edition: 1
  • Latest edition
  • Published: November 30, 2023
  • Language: English

About the editors

HD

Hirak Ranjan Dash

Hirak Ranjan Dash is a Researcher for the Forensic Science Laboratory at India’s National Institute of Technology. His expertise is in the field of environmental and forensic microbiology, DNA fingerprinting, microbial phylogeny and diversity, genetics, bioremediation, degradation of biological materials and applied microbiology. He has authored many publications, including articles, book chapters, and books.
Affiliations and expertise
Researcher, Forensic Science Laboratory, India’s National Institute of Technology, India

KE

Kelly M. Elkins

Dr. Elkins is Assistant Professor of Chemistry at Towson University where she teaches in the Masters of Science in Forensic Chemistry Program. Previously, she was the Director of Forensic Science and Assistant Professor of Chemistry at Metropolitan State College of Denver. She has her Bachelor of Science in Biology, Bachelor of Arts in Chemistry and her PhD in Chemistry with an emphasis on Biochemistry. Focused on the Forensic DNA field, she has developed a network of contacts in crime laboratories throughout Colorado and the Four Corners region and looks forward to extending her network in the Baltimore/DC area.
Affiliations and expertise
Assistant Professor of Chemistry, Chemistry Department and Forensic Chemistry Program, Towson University, Towson, Maryland, USA

NA

Noora Rashid Al-Snan

Dr. Noora Rashid Al-Snan is the Head of Biology/DNA Forensic Laboratory for the Directorate of Forensic Science, Ministry of Interior, Kingdom of Bahrain. She has a BSc degree in Molecular Biology –Biochemistry from Kuwait University, an MSc in Medical Biotechnology from Arabian Gulf University, and her PhD in Molecular Medicine from Arabian Gulf University specializing in Medical Genetics.
Affiliations and expertise
Head of Biology/DNA Forensic Laboratory, Directorate of Forensic Science, Ministry of Interior, Kingdom of Bahrain

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