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Nonisotopic DNA Probe Techniques

  • 1st Edition - March 30, 1992
  • Latest edition
  • Editor: Larry J. Kricka
  • Language: English

Recently many nonisotopic methods of probing specific DNA sequences have been developed as replacements for radioactive labels, such as 32phosphorous and 125iodine. This book… Read more

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Description

Recently many nonisotopic methods of probing specific DNA sequences have been developed as replacements for radioactive labels, such as 32phosphorous and 125iodine. This book brings all of these new methods together in one convenient, easily accessible source. It enables researchers to select the nonisotopic method best suited to their application and to use it to maximum advantage by following the straightforward instructions provided.
This book contains chapters on colorimetric, bioluminescent, chemiluminescent, fluorescent, and time-resolved fluorescent detection methods. Each chapter has been written by the inventor or developer of a particular nonisotopic method and thus provides an expert account of the method. Each chapterpresents useful background information and detailed, step-by-step, easy-to-follow, experimental procedures for labeling and detection.

Key features

Gives extensive practical informationCovers major types of nonisotopic labels and proceduresPresents background information for each methodProvides strategies and detailed experimental procedures for labeling and detecting DNA sequences byFluorescenceChemiluminescenceBioluminescenceColorimetry

Readership

Researchers in molecular biology, cell biology, genetics, developmental biology, mirobiology, clinical medicine, diagnostics, biochemistry, immunology, neurosciences, oncology, and pharmacology in academic and industrial laboratories.

Table of contents

(Section Headings): Introduction. Detection Methods. Index.

Product details

  • Edition: 1
  • Latest edition
  • Published: March 30, 1992
  • Language: English

About the editor

LK

Larry J. Kricka

Affiliations and expertise
Hospital of the University of Pennsylvania, Philadelphia, U.S.A.

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