Transketolases
- 1st Edition, Volume 722 - November 7, 2025
- Latest edition
- Editors: Bastien Doumèche, Franck Charmantray
- Language: English
Transketolase is an ubiquitous enzyme which has not yet finished revealing all its secrets. This Methods in Enzymology: Transketolases volume is devoted to the description of cut… Read more
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Description
Description
Transketolase is an ubiquitous enzyme which has not yet finished revealing all its secrets. This Methods in Enzymology: Transketolases volume is devoted to the description of cutting-edge methods to its study in biochemistry. Several topics are addressed by world experts in their field.
The first part of this volume deals with transketolase at the cellular level. The latest scientific progress on human transketolase TKL1 in relation to certain diseases and to its inhibition are discussed including the involvement of this enzyme in new metabolic pathways or as a diagnostic biomarker.
The second part focuses on transketolase at the molecular level. The structural and mechanistic aspects linked to this enzyme are addressed using several approaches including molecular modelling and crystallography, for example. Finally, the experimental methodological approaches relating to the use of transketolase as a tool of choice in biocatalysis is presented.
The first part of this volume deals with transketolase at the cellular level. The latest scientific progress on human transketolase TKL1 in relation to certain diseases and to its inhibition are discussed including the involvement of this enzyme in new metabolic pathways or as a diagnostic biomarker.
The second part focuses on transketolase at the molecular level. The structural and mechanistic aspects linked to this enzyme are addressed using several approaches including molecular modelling and crystallography, for example. Finally, the experimental methodological approaches relating to the use of transketolase as a tool of choice in biocatalysis is presented.
Key features
Key features
- Cutting-edge methodologies to study Transketolase from cellular to molecular contexts
- Detailed protocols described by selected experts and easily applicable to other enzymes
- A compilation of the methods for studying transketolases in a single volume
Readership
Readership
Enzymologists, Biochemists, Organic/Physical Chemists, Physicians, involved in metabolic pathways
studies, enzyme kinetics and enzyme mechanism elucidation, biocatalysis, chemical biology,
computational chemistry and molecular modeling
studies, enzyme kinetics and enzyme mechanism elucidation, biocatalysis, chemical biology,
computational chemistry and molecular modeling
Table of contents
Table of contents
1. Role of transketolase-like 1 in human cortical neurogenesis
Anneline Pinson and Wieland B. Huttner
2. Transketolase in metabolic diseases, autoimmune diseases and cancer
Xuemei Tong
3. Transketolase-like-1 (TKTL1) based one-substrate reaction and its impact on pathologies and treatments
Johannes Coy
4. Evaluation of binding, affinity and metabolic regulatory activity of a transketolase inhibitor
Xiaofei Chen
5. Discovery of novel transketolase epitopes and development of IgG-based tuberculosis serodiagnostics
Lobelia Samavati
6. Transketolases in apicomplexan parasites
Nishith Gupta
7. Electrochemical screening for microbial transketolase inhibitors identification
Chloé Aymard, Bastien Doumèche, Florian Bianco, Jean-François Chateaux, Franck Charmantray and Rainier-Numa Georges
8. Transketolases isolation from plants: Diversity of pathways
Dorothea Bartels
9. Transketolase-sulfoglycolytic pathway
Yan Zhang
10. Split-gene transketolase homolog that catalyzes the key step of biosynthetic pathway of phosphonothrixin in Saccharothrix sp.
Tomohisa Kuzuyama and Yunxun Zhu
11. Biophysical and structural analysis of transketolases
Kai Tittman and Fabian Rabe von Pappenheim
12. Structural variations of transketolases over the evolution from bacteria to higher eukaryotes: in silico/in vitro study
Rainier-Numa Georges, Bastien Doumèche, Lionel Ballut and Franck Charmantray
13. Structural and biochemical studies on split-gene transketolases, evolutionary aspects and comparisons
Jennifer Ann Littlechild
14. Transketolase catalytic reaction pathway. Study of ThDP activation and complete catalytic cycle
Vincent Thery and Lionel Nauton
15. Transketolases: Theoretical aspects from QM and QM/MM investigations
Tiziana Marino, Mario Prejanò and Nino Russo
16. Computational and structural insights into transketolase function and evolution: A detailed methodology
Thommas Mutemi Musyoka and Rita Afriyie Boateng
17. Transketolase enzyme discovery and the enzyme evolution of transketolase
Alessia Tonoli, Helen C. Hailes, Paul Dalby and Jack Jeffries
18. Evolved thermostable transketolase from Geobacillus stearothermophilus for deoxyketoses synthesis
Camille Gadona, Franck Charmantray, Laurence Hecquet, Giuseppe Arbia and Muriel Joly
19. Practical screening assays to identify the substrate scope of transketolase variants
Wolf-Dieter Fessner and Jules Perrard
20. Production of rare sugars by transketolase in combination with microbes and other enzymes
Akihide Yoshihara, Yoshida Hiromi, Ken Izumori and Shigehiro Kamitori
21. Engineered transketolase for cascade enzymatic synthesis with w-transaminase
Shuangjun Lin
22. Process considerations for the scale-up and implementation of transketolases
John Woodley
Anneline Pinson and Wieland B. Huttner
2. Transketolase in metabolic diseases, autoimmune diseases and cancer
Xuemei Tong
3. Transketolase-like-1 (TKTL1) based one-substrate reaction and its impact on pathologies and treatments
Johannes Coy
4. Evaluation of binding, affinity and metabolic regulatory activity of a transketolase inhibitor
Xiaofei Chen
5. Discovery of novel transketolase epitopes and development of IgG-based tuberculosis serodiagnostics
Lobelia Samavati
6. Transketolases in apicomplexan parasites
Nishith Gupta
7. Electrochemical screening for microbial transketolase inhibitors identification
Chloé Aymard, Bastien Doumèche, Florian Bianco, Jean-François Chateaux, Franck Charmantray and Rainier-Numa Georges
8. Transketolases isolation from plants: Diversity of pathways
Dorothea Bartels
9. Transketolase-sulfoglycolytic pathway
Yan Zhang
10. Split-gene transketolase homolog that catalyzes the key step of biosynthetic pathway of phosphonothrixin in Saccharothrix sp.
Tomohisa Kuzuyama and Yunxun Zhu
11. Biophysical and structural analysis of transketolases
Kai Tittman and Fabian Rabe von Pappenheim
12. Structural variations of transketolases over the evolution from bacteria to higher eukaryotes: in silico/in vitro study
Rainier-Numa Georges, Bastien Doumèche, Lionel Ballut and Franck Charmantray
13. Structural and biochemical studies on split-gene transketolases, evolutionary aspects and comparisons
Jennifer Ann Littlechild
14. Transketolase catalytic reaction pathway. Study of ThDP activation and complete catalytic cycle
Vincent Thery and Lionel Nauton
15. Transketolases: Theoretical aspects from QM and QM/MM investigations
Tiziana Marino, Mario Prejanò and Nino Russo
16. Computational and structural insights into transketolase function and evolution: A detailed methodology
Thommas Mutemi Musyoka and Rita Afriyie Boateng
17. Transketolase enzyme discovery and the enzyme evolution of transketolase
Alessia Tonoli, Helen C. Hailes, Paul Dalby and Jack Jeffries
18. Evolved thermostable transketolase from Geobacillus stearothermophilus for deoxyketoses synthesis
Camille Gadona, Franck Charmantray, Laurence Hecquet, Giuseppe Arbia and Muriel Joly
19. Practical screening assays to identify the substrate scope of transketolase variants
Wolf-Dieter Fessner and Jules Perrard
20. Production of rare sugars by transketolase in combination with microbes and other enzymes
Akihide Yoshihara, Yoshida Hiromi, Ken Izumori and Shigehiro Kamitori
21. Engineered transketolase for cascade enzymatic synthesis with w-transaminase
Shuangjun Lin
22. Process considerations for the scale-up and implementation of transketolases
John Woodley
Product details
Product details
- Edition: 1
- Latest edition
- Volume: 722
- Published: November 7, 2025
- Language: English
About the editors
About the editors
BD
Bastien Doumèche
Claude Bernard Lyon 1 and ICBMS (Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, UMR 5246 CNRS, France).After biochemistry and biology studies in Rouen (France), he obtained a master degree in Amiens on a biocatalysis topic, followed by a PhD at the RWTH-Aachen university (Germany) working on immobilized enzymes in two-phase systems under Prof. W. Hartemeier supervision. He joined the group of Dr. Rolland Furstoss and Dr. Alain Archelas at the CNRS in Marseille (France) for a post-doc on multi-gram scale biocatalysis using epoxide hydrolases. After a two-years temporary associate professor position at the university of Cergy-Pontoise (France) with Prof. Véronique Larreta-Garde on sol-gel transitions of protein-polysaccharides mixtures catalysed by enzymes, he became a lecturer at the University Lyon 1in 2006 where he developed original research in heterogeneous biocatalysis (ionic liquids) and more recently electrochemical screening assays using screen-printed electrodes for oxidoreductases (dehydrogenases, oxidases, laccases) and transketolases in collaboration with Dr. Franck Charmantray.
Affiliations and expertise
Claude Bernard Lyon 1 University, FranceFC
Franck Charmantray
Franck CHARMANTRAY is a researcher in Biocatalysis at the CNRS. He carries out his research work at the Institute of Chemistry in Clermont-Ferrand (ICCF, UMR6296, Clermont Auvergne University, France).
In 2001, he obtained a PhD in medicinal chemistry focusing on « A new family of DNA intercalating-alkylating agents » under the supervision of Dr. M. Demuynck, Grenoble 1 University, France. He then changed his research theme to focus on Studies on Homocitrate Synthase and other Lysine Pathway Enzymes during a postdoctoral internship funded by a BBRSC Fellowship, and carried out under the supervision of Prof. D. Young, at the University of Sussex, Brighton, UK. He returned to France to carry out a 2-year industrial postdoctoral internship in Biocatalysis financed by Laboratoires Fournier and which focused on the chemoenzymatic synthesis of antithrombotics. He was then recruited at the ICCF as a CNRS researcher in 2004. His research themes concern Biocatalysis, and in particular the study and optimization of transketolase by in vitro evolution for the synthesis of rare sugars and analogues in particular. He works in close collaboration with Dr. Bastien Doumèche with whom he recently developed an electrochemical screening for microbial transketolase inhibitors identification.
Affiliations and expertise
Researcher in Biocatalysis, CNRS, FranceView book on ScienceDirect
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